How Much BAC Water for Wolverine Stack?
Short answer: a 20 mg Wolverine Stack vial can be reconstituted with 1 to 4 mL of bacteriostatic water. The volume you pick does not change how much peptide is in the vial. It sets the concentration, so choose the volume that gives clean numbers for your protocol.
How much BAC water for a Wolverine Stack vial
BAC water is short for bacteriostatic water, which is water preserved with 0.9% benzyl alcohol. A Wolverine Stack vial holds 20 mg of lyophilized peptide in total, 10 mg of BPC-157 and 10 mg of TB-500. Any volume of BAC water that dissolves the powder will work. Two things guide the choice.
- Clean numbers. 2 mL of BAC water gives 10 mg per mL, or 10,000 mcg per mL, which makes the math simple.
- Measurable volumes. A more dilute solution makes each aliquot a larger volume, which is easier to measure accurately. A more concentrated solution makes each aliquot very small.
Concentration by volume of BAC water
| BAC water added | Concentration | Syringe units per 0.1 mL |
|---|---|---|
| 1 mL | 20 mg/mL (20,000 mcg/mL) | 10 units |
| 2 mL | 10 mg/mL (10,000 mcg/mL) | 10 units |
| 3 mL | 6.67 mg/mL (6,667 mcg/mL) | 10 units |
| 4 mL | 5 mg/mL (5,000 mcg/mL) | 10 units |
Units per 0.1 mL stays at 10 because that is a fixed property of a U-100 syringe. What changes is how much peptide sits in that 0.1 mL. Use the Wolverine Stack dosage and reconstitution calculator page to set any volume and aliquot with the slider.
How to reconstitute a lab vial step by step
- Bring the peptide vial and the BAC water to room temperature.
- Wipe both rubber stoppers with an alcohol swab and let them dry.
- Draw the chosen volume of BAC water into a sterile syringe.
- Insert the needle into the peptide vial and aim it at the inside wall of the glass.
- Add the water slowly so it runs down the wall and not directly onto the powder.
- Swirl the vial gently until the powder dissolves. Do not shake.
- Label the vial with the date and the concentration, and refrigerate it.
BAC water versus sterile water
Bacteriostatic water for Wolverine Stack
It contains 0.9% benzyl alcohol, which slows bacterial growth. That is why a vial of BAC water is designed for more than one needle entry in a laboratory setting.
Sterile water
It has no preservative. A vial is normally treated as single use, because once it is opened nothing in it stops bacteria from growing.
How long reconstituted Wolverine Stack lasts
No single number applies to every lab, because stability depends on temperature, light, how often the vial is entered and the cleanliness of the technique. The general practice is to keep the reconstituted solution at 2 to 8 C, away from light, and to use your own stability data or your institution's protocol to set how long it stays in use. Do not freeze and thaw it repeatedly. If the solution turns cloudy, changes color or has particles, discard it.
Supplies for reconstitution
Consumables
Alcohol swabs, sterile syringes of a suitable size, and sterile needles. Use a new needle and syringe for each entry.
Labels
A marker and labels for the date, the concentration and the diluent volume used.
Reconstitution mistakes to avoid
- Squirting water onto the powder. It can damage the peptide and foam the solution.
- Shaking the vial. Swirl it instead.
- Misreading units. On a U-100 syringe, 100 units is 1 mL, so 10 units is 0.1 mL.
- Not labeling the vial. A vial with no date and concentration is a source of errors.
- Using the label mass. Use the measured net content from the COA where you have it.
How much BAC water for Wolverine Stack: common questions
How much BAC water for Wolverine Stack if I use 3 mL on a 20 mg vial?
Yes. It gives about 6,667 mcg per mL. Any volume that dissolves the powder gives a valid concentration.
Can I add more BAC water later?
Yes, but then the concentration changes, so recalculate it and relabel the vial.
Does BAC water change how much peptide is in the vial?
No. The mass of peptide is fixed by the vial. BAC water only sets the concentration.
Is there a correct amount for a person?
This page covers laboratory concentration math and does not say what a person should use. No approved human amount exists.
Keep reading: Wolverine Stack dosage and reconstitution calculator, or how much Wolverine Stack should I inject.